DAPI Staining

1. Pellet 100-500 無 cells to be stained by spinning 30 sec at full speed in microfuge

2. Aspirate off media

3. Resuspend pellet in 300 無 PBS

4. Add 700 無 100% EtOH

5. Rock for 1 hour at RT to fix cells

6. Pellet cells by spinning and wash cells in 1 mL PBS

7. Resuspend pellet in 500 無 PBS

8. Add DAPI to 1 痢/mL (0.5 無 1 mg/mL DAPI)

9. Stain 5 min. by rocking at RT

10. Wash cells 2X in PBS

11. Resuspend pellet in 100-500 無 PBS

12. Place 5 無 cells and 1 無 mounting media on glass slide followed by a coverslip
(no need to seal the coverslip)

13. View cells on 60X